Long-term persistence of robust antibody and cytotoxic T cell responses in recovered patients infected with SARS coronavirus.
Identifieur interne : 003E27 ( Main/Exploration ); précédent : 003E26; suivant : 003E28Long-term persistence of robust antibody and cytotoxic T cell responses in recovered patients infected with SARS coronavirus.
Auteurs : Taisheng Li [République populaire de Chine] ; Jing Xie ; Yuxian He ; Hongwei Fan ; Laurence Baril ; Zhifeng Qiu ; Yang Han ; Wenbing Xu ; Weihong Zhang ; Hui You ; Yanling Zuo ; Qing Fang ; Jian Yu ; Zhiwei Chen ; Linqi ZhangSource :
- PloS one [ 1932-6203 ] ; 2006.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux (sang), Antigènes viraux (génétique), Données de séquences moléculaires, Facteurs temps, Femelle, Fréquence d'allèle, Gènes MHC de classe I, Gènes MHC de classe II, Humains, Immunoglobuline G (sang), Lymphocytes T cytotoxiques (immunologie), Mâle, Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Spécificité des anticorps, Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Séquence d'acides aminés, Virus du SRAS (génétique), Virus du SRAS (immunologie), Épitopes (génétique), Études cas-témoins, Études de suivi.
- MESH :
- génétique : Antigènes viraux, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS, Épitopes.
- immunologie : Lymphocytes T cytotoxiques, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Données de séquences moléculaires, Facteurs temps, Femelle, Fréquence d'allèle, Gènes MHC de classe I, Gènes MHC de classe II, Humains, Mâle, Spécificité des anticorps, Séquence d'acides aminés, Études cas-témoins, Études de suivi.
English descriptors
- KwdEn :
- Adult, Amino Acid Sequence, Antibodies, Viral (blood), Antibody Specificity, Antigens, Viral (genetics), Case-Control Studies, Epitopes (genetics), Female, Follow-Up Studies, Gene Frequency, Genes, MHC Class I, Genes, MHC Class II, Humans, Immunoglobulin G (blood), Male, Middle Aged, Molecular Sequence Data, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), T-Lymphocytes, Cytotoxic (immunology), Time Factors.
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Antigens, Viral, Epitopes, Nucleocapsid Proteins.
- chemical , immunology : Nucleocapsid Proteins.
- genetics : SARS Virus, Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Amino Acid Sequence, Antibody Specificity, Case-Control Studies, Female, Follow-Up Studies, Gene Frequency, Genes, MHC Class I, Genes, MHC Class II, Humans, Male, Middle Aged, Molecular Sequence Data, Time Factors.
Abstract
Most of the individuals infected with SARS coronavirus (SARS-CoV) spontaneously recovered without clinical intervention. However, the immunological correlates associated with patients' recovery are currently unknown. In this report, we have sequentially monitored 30 recovered patients over a two-year period to characterize temporal changes in SARS-CoV-specific antibody responses as well as cytotoxic T cell (CTL) responses. We have found persistence of robust antibody and CTL responses in all of the study subjects throughout the study period, with a moderate decline one year after the onset of symptoms. We have also identified two potential major CTL epitopes in N proteins based on ELISPOT analysis of pooled peptides. However, despite the potent immune responses and clinical recovery, peripheral lymphocyte counts in the recovered patients have not yet been restored to normal levels. In summary, our study has, for the first time, characterized the temporal and dynamic changes of humoral and CTL responses in the natural history of SARS-recovered individuals, and strongly supports the notion that high and sustainable levels of immune responses correlate strongly with the disease outcome. Our findings have direct implications for future design and development of effective therapeutic agents and vaccines against SARS-CoV infection.
DOI: 10.1371/journal.pone.0000024
PubMed: 17183651
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001F45
- to stream PubMed, to step Curation: 001F45
- to stream PubMed, to step Checkpoint: 002118
- to stream Ncbi, to step Merge: 001815
- to stream Ncbi, to step Curation: 001815
- to stream Ncbi, to step Checkpoint: 001815
- to stream Main, to step Merge: 004008
- to stream Main, to step Curation: 003E27
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Long-term persistence of robust antibody and cytotoxic T cell responses in recovered patients infected with SARS coronavirus.</title><author><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name><affiliation wicri:level="3"><nlm:affiliation>Department of Infectious Disease, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. litsh@263.net</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Disease, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xie, Jing" sort="Xie, Jing" uniqKey="Xie J" first="Jing" last="Xie">Jing Xie</name></author><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></author><author><name sortKey="Fan, Hongwei" sort="Fan, Hongwei" uniqKey="Fan H" first="Hongwei" last="Fan">Hongwei Fan</name></author><author><name sortKey="Baril, Laurence" sort="Baril, Laurence" uniqKey="Baril L" first="Laurence" last="Baril">Laurence Baril</name></author><author><name sortKey="Qiu, Zhifeng" sort="Qiu, Zhifeng" uniqKey="Qiu Z" first="Zhifeng" last="Qiu">Zhifeng Qiu</name></author><author><name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name></author><author><name sortKey="Xu, Wenbing" sort="Xu, Wenbing" uniqKey="Xu W" first="Wenbing" last="Xu">Wenbing Xu</name></author><author><name sortKey="Zhang, Weihong" sort="Zhang, Weihong" uniqKey="Zhang W" first="Weihong" last="Zhang">Weihong Zhang</name></author><author><name sortKey="You, Hui" sort="You, Hui" uniqKey="You H" first="Hui" last="You">Hui You</name></author><author><name sortKey="Zuo, Yanling" sort="Zuo, Yanling" uniqKey="Zuo Y" first="Yanling" last="Zuo">Yanling Zuo</name></author><author><name sortKey="Fang, Qing" sort="Fang, Qing" uniqKey="Fang Q" first="Qing" last="Fang">Qing Fang</name></author><author><name sortKey="Yu, Jian" sort="Yu, Jian" uniqKey="Yu J" first="Jian" last="Yu">Jian Yu</name></author><author><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name></author><author><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17183651</idno><idno type="pmid">17183651</idno><idno type="doi">10.1371/journal.pone.0000024</idno><idno type="wicri:Area/PubMed/Corpus">001F45</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F45</idno><idno type="wicri:Area/PubMed/Curation">001F45</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F45</idno><idno type="wicri:Area/PubMed/Checkpoint">002118</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002118</idno><idno type="wicri:Area/Ncbi/Merge">001815</idno><idno type="wicri:Area/Ncbi/Curation">001815</idno><idno type="wicri:Area/Ncbi/Checkpoint">001815</idno><idno type="wicri:Area/Main/Merge">004008</idno><idno type="wicri:Area/Main/Curation">003E27</idno><idno type="wicri:Area/Main/Exploration">003E27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Long-term persistence of robust antibody and cytotoxic T cell responses in recovered patients infected with SARS coronavirus.</title><author><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name><affiliation wicri:level="3"><nlm:affiliation>Department of Infectious Disease, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. litsh@263.net</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Disease, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xie, Jing" sort="Xie, Jing" uniqKey="Xie J" first="Jing" last="Xie">Jing Xie</name></author><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></author><author><name sortKey="Fan, Hongwei" sort="Fan, Hongwei" uniqKey="Fan H" first="Hongwei" last="Fan">Hongwei Fan</name></author><author><name sortKey="Baril, Laurence" sort="Baril, Laurence" uniqKey="Baril L" first="Laurence" last="Baril">Laurence Baril</name></author><author><name sortKey="Qiu, Zhifeng" sort="Qiu, Zhifeng" uniqKey="Qiu Z" first="Zhifeng" last="Qiu">Zhifeng Qiu</name></author><author><name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name></author><author><name sortKey="Xu, Wenbing" sort="Xu, Wenbing" uniqKey="Xu W" first="Wenbing" last="Xu">Wenbing Xu</name></author><author><name sortKey="Zhang, Weihong" sort="Zhang, Weihong" uniqKey="Zhang W" first="Weihong" last="Zhang">Weihong Zhang</name></author><author><name sortKey="You, Hui" sort="You, Hui" uniqKey="You H" first="Hui" last="You">Hui You</name></author><author><name sortKey="Zuo, Yanling" sort="Zuo, Yanling" uniqKey="Zuo Y" first="Yanling" last="Zuo">Yanling Zuo</name></author><author><name sortKey="Fang, Qing" sort="Fang, Qing" uniqKey="Fang Q" first="Qing" last="Fang">Qing Fang</name></author><author><name sortKey="Yu, Jian" sort="Yu, Jian" uniqKey="Yu J" first="Jian" last="Yu">Jian Yu</name></author><author><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name></author><author><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Amino Acid Sequence</term><term>Antibodies, Viral (blood)</term><term>Antibody Specificity</term><term>Antigens, Viral (genetics)</term><term>Case-Control Studies</term><term>Epitopes (genetics)</term><term>Female</term><term>Follow-Up Studies</term><term>Gene Frequency</term><term>Genes, MHC Class I</term><term>Genes, MHC Class II</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (genetics)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (génétique)</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Femelle</term><term>Fréquence d'allèle</term><term>Gènes MHC de classe I</term><term>Gènes MHC de classe II</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Mâle</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère (génétique)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Épitopes (génétique)</term><term>Études cas-témoins</term><term>Études de suivi</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Amino Acid Sequence</term><term>Antibody Specificity</term><term>Case-Control Studies</term><term>Female</term><term>Follow-Up Studies</term><term>Gene Frequency</term><term>Genes, MHC Class I</term><term>Genes, MHC Class II</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Femelle</term><term>Fréquence d'allèle</term><term>Gènes MHC de classe I</term><term>Gènes MHC de classe II</term><term>Humains</term><term>Mâle</term><term>Spécificité des anticorps</term><term>Séquence d'acides aminés</term><term>Études cas-témoins</term><term>Études de suivi</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most of the individuals infected with SARS coronavirus (SARS-CoV) spontaneously recovered without clinical intervention. However, the immunological correlates associated with patients' recovery are currently unknown. In this report, we have sequentially monitored 30 recovered patients over a two-year period to characterize temporal changes in SARS-CoV-specific antibody responses as well as cytotoxic T cell (CTL) responses. We have found persistence of robust antibody and CTL responses in all of the study subjects throughout the study period, with a moderate decline one year after the onset of symptoms. We have also identified two potential major CTL epitopes in N proteins based on ELISPOT analysis of pooled peptides. However, despite the potent immune responses and clinical recovery, peripheral lymphocyte counts in the recovered patients have not yet been restored to normal levels. In summary, our study has, for the first time, characterized the temporal and dynamic changes of humoral and CTL responses in the natural history of SARS-recovered individuals, and strongly supports the notion that high and sustainable levels of immune responses correlate strongly with the disease outcome. Our findings have direct implications for future design and development of effective therapeutic agents and vaccines against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Baril, Laurence" sort="Baril, Laurence" uniqKey="Baril L" first="Laurence" last="Baril">Laurence Baril</name><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name><name sortKey="Fan, Hongwei" sort="Fan, Hongwei" uniqKey="Fan H" first="Hongwei" last="Fan">Hongwei Fan</name><name sortKey="Fang, Qing" sort="Fang, Qing" uniqKey="Fang Q" first="Qing" last="Fang">Qing Fang</name><name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><name sortKey="Qiu, Zhifeng" sort="Qiu, Zhifeng" uniqKey="Qiu Z" first="Zhifeng" last="Qiu">Zhifeng Qiu</name><name sortKey="Xie, Jing" sort="Xie, Jing" uniqKey="Xie J" first="Jing" last="Xie">Jing Xie</name><name sortKey="Xu, Wenbing" sort="Xu, Wenbing" uniqKey="Xu W" first="Wenbing" last="Xu">Wenbing Xu</name><name sortKey="You, Hui" sort="You, Hui" uniqKey="You H" first="Hui" last="You">Hui You</name><name sortKey="Yu, Jian" sort="Yu, Jian" uniqKey="Yu J" first="Jian" last="Yu">Jian Yu</name><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name><name sortKey="Zhang, Weihong" sort="Zhang, Weihong" uniqKey="Zhang W" first="Weihong" last="Zhang">Weihong Zhang</name><name sortKey="Zuo, Yanling" sort="Zuo, Yanling" uniqKey="Zuo Y" first="Yanling" last="Zuo">Yanling Zuo</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003E27 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003E27 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17183651
|texte= Long-term persistence of robust antibody and cytotoxic T cell responses in recovered patients infected with SARS coronavirus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17183651" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |